Long non-coding RNA LINC00470 regulates the growth and epithelial-to-mesenchymal transition of human oral cancer cells

Purpose: To investigate the role of long non-coding RNA LINC00470 in human oral cancer tissues and cell lines (OC3, SCC9 CAL27).
 Methods: Expression mRNA was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Cell viability proliferation were measured using MTT EdU assays, respectively, while migration invasion with transwell assay. Protein expression assayed western blotting.
 Results: There significant up-regulation CAL27) (p < 0.05). Silencing led to inhibition colony-formation potential cells Furthermore, silencing caused induction apoptosis, suppression invasion, restoration epithelial-to-mesenchymal transition (EMT).
 Conclusion: These results strongly demonstrate oncogenic cancer, thereby suggesting its possible prognostic therapeutic this lethal malignancy.